Joekai Biotechnology LLC

Your CNS Service & Health Provider

zzhuo

Preclinical CRO Services

Phenotypic Screening for CNS Disorders

    Drug discovery in CNS disorders has becoming one of the most difficult and costly franchises in modern pharmaceutical research. Many CNS disorders are too complicated to be narrowed down to the single target for mechanisms, therefore limiting the benefits of target- and cell-based approaches before pharmacological validation in animal models. Joekai’s “phenotypic in vivo screening” services with fly models provide direct indication of clinical-relevant behaviors in high-throughput manner. As screened out at entire organ level, drug candidates are having fewer concerns on ADMET issues. Feel free to contact us for the interests regarding to the services provided below, and the most current updates
(contact us).

Alzheimer's disease (AD)

    Human AD patients suffer progressive dementia problems. A phenotypic screening directly at learning & memory behavior level will be an excellent strategy to start with for AD drug discovery projects. We offers professional services to evaluate the resuce of learning and memory deficit caused by drug candidates in massive screening scale with fruitflies, and in further pharmacological validation with rodents. Other useful assays in fly system (e.g., pathological brain section, gene profiling, and etc...) are also provided in below: 

Fly A-beta 42 model

Fly tau-hyperphosphorylation model

Mouse double K.O. model

Memory test

Locomotion test

 

Life span assay

 

Brain sectioning for pathology

 

 

Stress tests

 

 

Gene profiling

 

 


Parkinson’s disease (PD)

    
Unlike AD, human PD patients are characterized with locomotion deficits and uncontrolled movement. We have adapted climbing and flying tests in flies for a better accessment during the initial screening for PD drugs. Due to the known pathological sites and mechanizms for PD, the brain section images of dopaminergic (DA) neurons and oxidative stress tests are also included. See below table and contact us for details.

Fly Pink model

Fly Parkin model

Other fly loss-of-function model

Memory test

Climbing test

Courtship assay

 

Pathology section on DA neurons

 

 

Oxidative stress tests

 

 

Autism Spectrum Disorder (ASD)

Fly FMR1* model

Fly neuroligin model

Other fly loss-of-function model

Reverse memory test

 

 

 

Repeated behavior test

 

 

 

Courtship assay

 

 

 

 

 

 

 

 

 

* Fragile X mental retardation 1

Schizophrenia

Fly Dysbindin model

Fly DISC1 model

Other fly loss-of-function model

Memory test

 

Locomotor activity

 

Mating preference